Thermo Fisher Scientific LEMD2 Polyclonal Antibody
Human LEMD2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. 연구용으로 핵 구조 및 근육 질환 관련 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human LEMD2 (Product #RP-89512) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2643336 |
Product Specific Information
Immunogen sequence:
EDNMKLLPVD CERKTDEFCQ AKQKAALLEL LHELYNFLAI QAGNFECGNP ENLKSKCIPV MEAQEYIANV TSSSSAKFEA ALTWILSSNK DVGIWLKGED QSELVTTVDK VVCLESAHP
Highest antigen sequence identity to orthologs:
- Mouse: 92%
- Rat: 90%
Target Information
The LEM (lamina-associated polypeptide-emerin-MAN1) domain is a motif shared by lamin-interacting proteins of the inner nuclear membrane (INM) and nucleoplasm.
LEMD2 (LEM domain-containing protein 2) is a lamina-associated protein in the INM involved in nuclear structure organization. It contains an N-terminal LEM motif, two transmembrane domains, and a MAN1-Src1p C-terminal (MSC) domain.
LEMD2 is a truncated paralog of MAN1, lacking the MAN1-specific RNA-recognition motif, but is required for myogenic differentiation. Mutations in LEMD2 may contribute to muscle disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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