Thermo Fisher Scientific Phospho-14-3-3 zeta (Thr232) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000
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Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:100
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Product Specifications

Target Information

At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins.

By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor.

Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.