Thermo Fisher Scientific CD11b/MAC-1 Monoclonal Antibody (ITGAM, 3338)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95 °C, followed by cooling at room temperature for 20 minutes.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3).

CD11b is expressed on:

• Monocytes/macrophages
• Granulocytes
• Activated lymphocytes
• A subset of NK cells and dendritic cells
• Microglia in the brain

The CD11b/CD18 complex functions as a receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. These interactions are critical for cell–cell and cell–matrix adhesion.

CD11b is expressed on approximately:

• 8% of spleen cells
• 44% of bone marrow cells
• <1% of thymocytes

It is widely used as a microglial marker in nervous tissue. Expression increases during monocyte maturation and varies among tissue macrophages; peritoneal macrophages express higher levels than splenic macrophages.

Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.