Thermo Fisher Scientific SARS-CoV-2 NSP1 Polyclonal Antibody
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SARS-CoV-2 NSP1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, ELISA, IP에 사용 가능. 고순도 Affinity chromatography 정제, 액상 형태로 보관. 연구용으로만 사용.
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Thermo Fisher Scientific SARS-CoV-2 NSP1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| ELISA | 1 µg/mL | – |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–180 of coronavirus NSP1 (YP_0097426081) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.76 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901571 |
Product Specific Information
- Positive Samples: 293T
- Immunogen Sequence:
MESLVPGFNE KTHVQLSLPV LQVRDVLVRG FGDSVEEVLS EARQHLKDGT CGLVEVEKGV LPQLEQPYVF IKRSDARTAP HGHVMVELVA ELEGIQYGRS GETLGVLVPH VGEIPVAYRK VLLRKNGNKG AGGHSYGADL KSFDLGDELG TDPYEDFQEN WNTKHSSGVT RELMRELNGG
Target Information
- Inhibits host translation by interacting with the 40S ribosomal subunit (PubMed:32680882).
- Nsp1 C terminus binds to and obstructs ribosomal mRNA entry tunnel, inhibiting antiviral response triggered by innate immunity or interferons.
- The nsp1–40S ribosome complex induces endonucleolytic cleavage near the 5' UTR of host mRNAs, leading to degradation.
- Viral mRNAs are protected from degradation due to the presence of a 5'-end leader sequence.
- By suppressing host gene expression, nsp1 facilitates efficient viral gene expression and evasion from host immune response.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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