Thermo Fisher Scientific PKM2 Recombinant Rabbit Monoclonal Antibody (3H4L19)
Recombinant rabbit monoclonal antibody targeting human PKM2 (clone 3H4L19). Validated for Western blot at 1:5,000 dilution. Offers high specificity, lot-to-lot consistency, and broad cross-reactivity. Suitable for research use only.
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Applications
Tested Application
- Western Blot (WB): 1:5,000 dilution
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3H4L19 |
| Immunogen | Peptides corresponding to human PKM2 (aa476–aa493, aa120–aa136) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2784581 |
Additional Formats
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Pig, and Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
PKM2 (Pyruvate Kinase M2) catalyzes the conversion of phosphoenolpyruvate to pyruvate and ATP. It interacts with thyroid hormone, mediating metabolic effects, and binds Opa protein, implicating it in bacterial pathogenesis.
Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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