Thermo Fisher Scientific ENG Monoclonal Antibody (OTI3H5), TrueMAB
Human ENG (CD105/Endoglin) 단백질을 인식하는 마우스 모노클로날 항체로, WB, IHC, ICC 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제, 보존제 무첨가, 동결건조 형태로 안정적 저장 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H5 |
| Immunogen | Full length human recombinant protein of human ENG (CD105/Endoglin, UniProt ID: P17813-1, range: 1–658) produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CD105 (Endoglin) is a Type I transmembrane protein highly expressed on human vascular endothelial cells. It forms an O- and N-glycosylated homodimer. Upregulation of endoglin expression is observed in tumor vasculature and proliferating cells, indicating its role as a proliferation-associated endothelial marker. CD105 binds TGF beta 1 and 3 with high affinity but not TGF beta 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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