Thermo Fisher Scientific GUCA1B Monoclonal Antibody (5E7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

ELISA (ELISA)

0.1 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

5E7

Immunogen

GUCA1B (NP_002089, 93 a.a. approximately 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GCAP2,uniProtId:Q9UMX6-1,ncbiNodeId:9606,antigenRange:93-200,antigenLength:200,antigenImageFileName:H00002979-M01_GCAP2_Q9UMX6-1_House_mouse.svg,antigenImageFileNamePDP:H00002979-M01_GCAP2_Q9UMX6-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: KLKWTFKIYD KDGNGCIDRL ELLNIVEGIY QLKKACRREL QTEQDQLLTP EEVVDRIFLL VDENGDGQLS LNEFVEGARR DKWVMKMLQM DMNPSSWLAQ QRRKSAMF

Target Information

Guanylate cyclase-activating proteins (GCAPs) are calcium binding proteins that belong to the calmodulin superfamily. GCAPs without calcium are responsible for activation of photoreceptor guanylate cyclase during light adaptation. Studies have shown that the addition or subtraction of Ca2+ results in major conformational changes and the activation/deactivation of GCAP-1 and GCAP-2. It has been demonstrated that both GCAP-1 and 2 act on guanylate cyclase similarly and have approximately 50% homology between the two forms. GCAP-1 is predominantly localized in the photoreceptor outer segments, while GCAP-2 is found in retina samples.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.