Thermo Fisher Scientific Phospho-NSE (Tyr236) Polyclonal Antibody
Rabbit polyclonal antibody targeting phosphorylated NSE (Tyr236). Validated for Dot blot. Predicted cross-reactivity with mouse and rat. Suitable for detecting neuronal and neuroendocrine cells. Supplied in liquid form, purified by Protein A and antige...
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Applications
- Dot blot (DB): Tested dilution 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y236 of human NSE |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A, Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2099200 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
Neuron specific enolase (NSE, also known as ENO1, ENO2, ENO3 or enolase alpha, beta, gamma) catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. NSE is stable in biological fluids and diffuses into cerebrospinal fluid (CSF) when neuronal membranes are injured. It is expressed primarily in neurons and neuroendocrine cells, often co-expressed with chromogranin A in neuroendocrine neoplasms.
Antibodies to NSE are useful for identifying neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells. Release of NSE into CSF and blood serves as a biomarker of neuronal injury and is clinically used as a marker in neurological disorders such as stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Elevated NSE levels are also observed in certain neoplasias, including pediatric neuroblastoma and small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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