Thermo Fisher Scientific ATG12 Polyclonal Antibody
ATG12 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 사용 가능. 인간, 생쥐, 랫트 반응성. 고순도 항원 친화 크로마토그래피 정제. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (IHC) | 2.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 16 amino acid peptide from near the amino terminus of human ATG12 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage store at -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152882 |
Product Specific Information
- Suggested positive control: human brain tissue lysate
- PA5-20463 can be used with blocking peptide PEP-0580.
Target Information
ATG12 is a member of the autophagy protein family that forms a conjugate with ATG5, exhibiting ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy.
This conjugate associates with innate immune response proteins such as RIG-I and VISA (IPS-1), inhibiting type I interferon production and allowing viral replication in host cells.
ATG12 also interacts with ATG10 in human embryonic kidney cells in the presence of ATG7.
At least two isoforms of ATG12 are known to exist.
Autophagy, a process of bulk degradation of cellular proteins via the autophagosomic-lysosomal pathway, is crucial for normal growth and may be defective in tumor cells.
It helps preserve cellular nutrients under starvation and regulates turnover of cytosolic components.
This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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