Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), NovaFluor Blue 585, eBioscience

Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), NovaFluor Blue 585

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (0.6 µg)/test
  View 1 publication

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510, 555, 610-30S, 660-40S, 800
• NovaFluor Yellow 570, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, interfollicular macrophages, and some dendritic cells. CD14 binds LPS-LBP complexes with high affinity on monocyte surfaces.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated on normal human peripheral blood cells at 5 µL (0.6 µg)/test.
A test is defined as staining a cell sample (10⁵–10⁸ cells) in 100 µL final volume.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for minimal spectral spillover and spread impact.
Excitation: 494 nm; Emission: 585 nm; Laser: 488 nm (Blue).

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on monocytes, macrophages, and neutrophils. It functions as a lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 interacts with LPS-binding protein (LBP) and plays roles in immune recognition, inflammation, and apoptosis.
Associated diseases include Mycobacterium chelonae infection and Croup.
Alternative splicing produces multiple transcript variants of the same isoform.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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