Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Biotin
Thermo Fisher Scientific의 Donkey anti-Rat IgG (H+L) Biotin 결합 2차 항체로, Rat IgG 검출에 최적화된 고도로 교차흡착 제품입니다. Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능하며, 높은 특이성과 낮은 비특이적 결합을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:50,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535526 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit and sheep IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- AP
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of target molecules. Secondary antibodies enhance versatility by allowing the use of various detection systems (e.g., HRP, AP, fluorescence) and improve sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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