Thermo Fisher Scientific PPP3CA Polyclonal Antibody
Rabbit polyclonal antibody targeting human PPP3CA (Calcineurin A). Validated for WB, IHC, IHC(P), and IP applications. Recommended dilution range: 1:1,000–1:10,000 depending on assay. Predicted cross-reactivity with rat and bovine species. For ...
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Thermo Fisher Scientific PPP3CA Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 470 and the C-terminus (residue 521) of Human Protein Phosphatase 3, Catalytic Subunit, Alpha isoform (Calcineurin A, UniProt ID: Q08209-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat and Bovine
Target Information
Calcineurin plays an important role in the T-cell receptor-mediated signal transduction pathway.
The protein encoded by this gene binds specifically to the activated form of calcineurin and inhibits calcineurin-mediated signal transduction.
It is localized in the nucleus and contains a leucine zipper domain and several PEST motifs, which confer targeted degradation.
Alternative splicing results in multiple transcript variants encoding two different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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