Thermo Fisher Scientific Alexa Fluor 488 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 10 publications 10 publications

Immunohistochemistry (IHC)

Assay-dependent

View 45 publications 45 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 53 publications 53 publications

Flow Cytometry (Flow)

Assay-dependent

View 17 publications 17 publications

ELISA (ELISA)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 4 publications 4 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

Neutralization (Neu)

-

View 2 publications 2 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

Peptide Array (Array)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 6 publications 6 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Dog, Fruit fly, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Alexa Fluor 488 coupled to carrier protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_221544

Target Information

Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes. The Alexa Fluor® 488 dye possesses near identical spectral properties and quantum yield to fluorescein isothiocyanate (FITC), but produces brighter, more photostable conjugates. These conjugates are used for imaging and other applications requiring increased sensitivity without being overly impacted by environmental factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.