Thermo Fisher Scientific IQSEC1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

2 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

19 amino acid peptide near the amino terminus of human IQSEC1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IQSEC1,uniProtId:Q6DN90-1,ncbiNodeId:9606,antigenRange:1,antigenLength:963,antigenImageFileName:PA5-38019_IQSEC1_Q6DN90-1_Rabbit.svg,antigenImageFileNamePDP:PA5-38019_IQSEC1_Q6DN90-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2554623

Product Specific Information

A recommended positive control is A-20 cell lysate.

At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is not expected to cross-react with IQSEC2.

Target Information

ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain. As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis. IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.