Thermo Fisher Scientific Phospho-Akt (Ser473) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (IHC)

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence that includes phosphorylation sites of Serine 473 (Q-F-S(p)-Y-S) derived from human Akt and conjugated to KLH. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKT1,uniProtId:P31749-1,ncbiNodeId:9606,antigenRange:471-475,antigenLength:480,antigenImageFileName:43054-100UL_AKT1_P31749-1_Rabbit.svg,antigenImageFileNamePDP:43054-100UL_AKT1_P31749-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:AKT1,uniProtId:P47196-1,ncbiNodeId:10114,antigenRange:473,antigenLength:480,antigenImageFileName:43054-100UL_AKT1_P47196-1_Rabbit.svg,antigenImageFileNamePDP:43054-100UL_AKT1_P47196-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

Dulbecco`s PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.