Thermo Fisher Scientific Sp1 Polyclonal Antibody
Rabbit polyclonal antibody targeting SP1 transcription factor. Suitable for WB, IHC, IP, and ChIP-Seq applications. Recognizes region 750–785 of SP1. Supplied as liquid, unconjugated, 0.2 mg/mL concentration. Stored at 4°C, shipped on wet ice.
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Thermo Fisher Scientific Sp1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
| ChIP-sequencing (ChIP-Seq) | 4 µg/30 µg chromatin |
Product Specifications
| Property | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 750 and the C-terminus (residue 785) of SP1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in cell growth, apoptosis, differentiation, and immune responses.
SP1 activity is tightly regulated by post-translational modifications such as phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation. It also binds the PDGFR-alpha G-box promoter and may play a role in modulating cellular responses to DNA damage and chromatin remodeling.
SP1 is essential for the recruitment of SMARCA4/BRG1 on the c-FOS promoter and for the regulation of FE65 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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