Thermo Fisher Scientific MBD2 Recombinant Rabbit Monoclonal Antibody (4D4D5)
MBD2 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 ELISA에 적합합니다. HEK293 세포에서 발현되었으며, 고순도의 Affinity Chromatography 정제 방식으로 제조되었습니다. PBS 버퍼에 보관되며 -20°C에서 안정적으로 저장 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4D4D5 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 100–200 of human MBD2 (Q9UBB5). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.18 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911985 |
Product Specific Information
- Positive test controls include: Mouse liver.
- Immunogen sequence:
GGSGLGGDGG GCGGGGSGGG GAPRREPVPF PSGSAGPGPR GPRATESGKR MDCPALPPGW KKEEVIRKSG LSAGKSDVYY FSPSGKKFRS KPQLARYLGN T
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. Mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, which are heterochromatic adaptor molecules involved in gene silencing and chromatin structure organization. High-affinity recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain, serving as a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-primary mouse fibroblasts but restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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