Thermo Fisher Scientific Human CD155 (PVR) His-tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human CD155 isoform Alpha, amino acids Met1-Asn343 (Accession # P15151-1) with a C-terminal His-tag

Tag

His-tag

Molecular weight

36.5 kDa

Class

Recombinant

Type

Protein

Purity

≥97 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.

Endotoxin concentration

<1.0 EU/µg

Activity

1.Measured by its binding ability in a functional ELISA. Immobilized Human CD155 (PVR) His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind human DNAM1, the EC50 of human DNAM1/CD226 is 50-300 ng/mL.
2.Loaded Human CD226 Protein, hFc Tag on ProA Biosensor, can bind Human CD155 (PVR) His-tag Recombinant Protein with an affinity constant of 0.87 µM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested).
3.Loaded Recombinant Human TIGIT Protein, hFc Tag on ProA Biosensor, can bind Human CD155 (PVR) His-tag Recombinant Protein with an affinity constant of 0.18 uM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.