Thermo Fisher Scientific Human CD155 (PVR) His-tag Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• Immunohistochemistry (IHC): Assay-dependent
• ELISA: Assay-dependent
• Functional Assay: Assay-dependent

Product Specifications

Activity

1. Functional ELISA: Immobilized Human CD155 (PVR) His-tag Recombinant Protein at 2 µg/mL (100 µL/well) binds human DNAM1 with EC₅₀ of 50–300 ng/mL.
2. BLI Assay (DNAM1/CD226): Human CD226 Protein (hFc Tag) binds Human CD155 (PVR) His-tag Recombinant Protein with affinity constant of 0.87 µM (Sartorius Octet Red384).
3. BLI Assay (TIGIT): Recombinant Human TIGIT Protein (hFc Tag) binds Human CD155 (PVR) His-tag Recombinant Protein with affinity constant of 0.18 µM (Sartorius Octet RED384).

Product Specific Information

Reconstitution:
Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower.
It is recommended to aliquot and use as soon as possible.
Store aliquots under sterile conditions at -20°C.
Avoid repeated freeze-thaw cycles.

Target Information

The CD155 (PVR) gene encodes a transmembrane glycoprotein of the immunoglobulin superfamily.

• The extracellular domain mediates cell attachment to the extracellular matrix molecule vitronectin.
• The intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1.
• It serves as a cellular receptor for poliovirus in the first step of poliovirus replication.
• Multiple transcript variants encoding different isoforms have been identified.
• This gene is specific to the primate lineage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.