Thermo Fisher Scientific Enterokinase Monoclonal Antibody (5E3C8)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

-

ELISA (ELISA)

0.05-0.2 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5E3C8

Immunogen

Purified recombinant light chain of bovine EK protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.

Enterokinase is an intestinal enzyme responsible for initiating activation of pancreatic proteolytic proenzymes. Mouse Anti-Enterokinase Monoclonal Antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant EK protein (bovine EK, aa848-1035, light chain, 93% homologous with human EK).Mouse Anti-Enterokinase Monoclonal Antibody recognizes EK in Western blots and ELISAs.

Target Information

This gene encodes an enzyme that converts the pancreatic proenzyme trypsinogen to trypsin, which activates other proenzymes including chymotrypsinogen and procarboxypeptidases. The precursor protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. This protein is a member of the trypsin family of peptidases. Mutations in this gene cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive. [provided by RefSeq, Jul 2008].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.