Thermo Fisher Scientific SMURF2 Polyclonal Antibody
SMURF2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC(P)에서 검증됨. Human, Mouse, Rat에 반응하며 항원 친화 크로마토그래피로 정제됨. E3 유비퀴틴 리가아제 연구용으로 적합. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2. The immunogen is located within amino acids 140–190 of SMURF2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2890501 |
Product Specific Information
Positive controls that may be used include: C2C12 Cell Lysate
Target Information
SMURF2 is an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers it to targeted substrates. It interacts with SMAD1, SMAD2, and SMAD7 to trigger their ubiquitination and proteasome-dependent degradation. SMURF2 enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression with SMAD1 decreases SMAD1 protein levels and slightly reduces SMAD2 levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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