Thermo Fisher Scientific Cytokeratin Pan Antibody Cocktail

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:200

View 13 publications 13 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fish, Guinea pig, Human, Mouse, Pig

Host/Isotype

Mouse / IgG, kappa

Class

Cocktail

Type

Antibody

Clone

PAN-CK

Immunogen

Human epidermal keratins

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10942225

Product Specific Information

This antibody is a broad-spectrum pan-keratin monoclonal antibody cocktail which recognizes keratin 4, 5, 6, 7, 8, 10, 13, 14, 18 and 19. This product can differentiate between epithelial and non-epithelial tumors.

For staining of formalin-fixed tissue,digest sections wtih trypsin at 1 mg/mL in PBS, 10 min at 37ºC, or Protease XXV at 1 mg/mL in PBS for 5 min at 37ºC.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.