Thermo Fisher Scientific Phospho-ATM (Ser1981) Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

This antibody detects endogenous levels of ATM only when phosphorylated at serine 1981.

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein belonging to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and increased risk of lymphoid cancer. The ATM gene encodes a protein within the phosphoinositide 3-kinase (PI3K) superfamily.

ATM phosphorylates proteins rather than lipids and regulates cell-cycle-related targets such as P53, Mdm2, BRCA1, and SMC1. It plays a critical role in repairing double-stranded DNA breaks caused by ionizing radiation and other mutagens. The C-terminal region of ATM shares extensive homology with the catalytic domains of PI3 kinases. Studies have shown that ATM becomes autophosphorylated and upregulated upon exposure to ionizing radiation. AT cells are hypersensitive to radiation, impaired in DNA synthesis inhibition, and show delayed p53 induction. DNA damage activates ATM-kinase, triggering a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA repair. ATM is also linked to apoptosis via Nbs1 and Chk2 in the E2F1 pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.