Thermo Fisher Scientific TRIM28 Polyclonal Antibody
TRIM28 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC(P), ICC/IF에 적합합니다. KLH 결합 합성 펩타이드(178-207 aa, N-말단)로 면역화되었으며, Human 시료에 반응합니다. Protein A 및 항원 친화 크로마토그래피로 정제된 액상 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 178–207 amino acids from the N-terminal region of human TRIM28 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.45 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2552389 |
Target Information
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription via interactions with KAP-1 (KRAB-associated protein 1).
KAP-1 is an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger. It forms complexes with KRAB-domain transcription factors to enhance transcriptional repression. KAP-1 also interacts with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1), targeting KRAZ1 to centromeric heterochromatin regions containing HP1α, thereby regulating transcriptional repression. Mutations in KAP-1 that disrupt HP1 binding lead to random KRAZ1 distribution and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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