Thermo Fisher Scientific JMJD2B Recombinant Rabbit Monoclonal Antibody (ARC1416)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

ChIP assay (ChIP)

5 µg antibody for 10 µg-15 µg of Chromatin

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC1416

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KDM4B/JMJD2B (O94953) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:JMJD2B,uniProtId:O94953-1,ncbiNodeId:9606,antigenRange:50-150,antigenLength:1096,antigenImageFileName:MA5-35692_JMJD2B_O94953-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35692_JMJD2B_O94953-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849592

Product Specific Information

Immunogen sequence: PPKEWKPRQT YDDIDDVVIP APIQQVVTGQ SGLFTQYNIQ KKAMTVGEYR RLANSEKYCT PRHQDFDDLE RKYWKNLTFV SPIYGADISG SLYDDDVAQW N

Target Information

Histone demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 Lys-4, H3 Lys-27, H3 Lys-36 nor H4 Lys-20. Only able to demethylate trimethylated H3 Lys-9, with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.