Thermo Fisher Scientific JMJD2B Recombinant Rabbit Monoclonal Antibody (ARC1416)
JMJD2B 단백질을 표적으로 하는 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot, ICC/IF, ELISA, IP, ChIP 등 다양한 응용에 적합. 높은 특이성과 재현성을 제공하며 HEK293 세포에서 발현된 IgG 형 항체. 연구용으로만 사용 가능.
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Thermo Fisher Scientific JMJD2B Recombinant Rabbit Monoclonal Antibody (ARC1416)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
| ChIP assay (ChIP) | 5 µg antibody for 10–15 µg of chromatin |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1416 |
| Immunogen | Synthetic peptide corresponding to amino acids 50–150 of human KDM4B/JMJD2B (UniProt ID: O94953) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849592 |
Product Specific Information
Immunogen sequence:
PPKEWKPRQT YDDIDDVVIP APIQQVVTGQ SGLFTQYNIQ KKAMTVGEYR RLANSEKYCT PRHQDFDDLE RKYWKNLTFV SPIYGADISG SLYDDDVAQW N
Target Information
Histone demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a role in the histone code.
Does not demethylate histone H3 Lys-4, Lys-27, Lys-36, nor H4 Lys-20.
Only demethylates trimethylated H3 Lys-9, with weaker activity than KDM4A, KDM4C, and KDM4D.
Demethylation generates formaldehyde and succinate.
[UniProt]
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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