Thermo Fisher Scientific UBE2C Monoclonal Antibody (3B1)
UBE2C 단백질을 인식하는 Mouse monoclonal antibody(클론 3B1). Western blot, ICC/IF, ELISA에 최적화. 인간 시료 반응성. PBS buffer, no preservative, -20°C 보관. 연구용으로 세포주기 및 APC/C 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3B1 |
| Immunogen | UBE2C (AAH50736, 1 a.a. ~179 a.a) full-length recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein sequence:
MASQNRDPAA TSVAAARKGA EPSGGAARGP VGKRLQQELM TLMMSGDKGI SAFPESDNLF KWVGTIHGAA GTVYEDLRYK LSLEFPSGYP YNAPTVKFLT PCYHPNVDTQ GNICLDILKE KWSALYDVRT ILLSIQSLLG EPNIDSPLNT HAAELWKNPT AFKKYLQETY SKQVTSQEP
Target Information
The UBE2C gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is essential for mitotic cyclin destruction and cell cycle progression and may be implicated in cancer progression. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, it catalyzes Lys-11- and Lys-48-linked polyubiquitination. UBE2C acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls mitotic progression by initiating Lys-11-linked polyubiquitin chains on APC/C substrates, leading to their degradation by the proteasome and promoting mitotic exit.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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