Thermo Fisher Scientific DLAT Polyclonal Antibody
DLAT 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, ICC/IF, ELISA에 적합합니다. 인체, 마우스, 랫트 반응성이 있으며, 고순도 Affinity Chromatography로 정제되었습니다. -20°C 보관, 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:20–1:50 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–290 of human DLAT (NP_001922.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.16 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855048 |
Product Specific Information
Immunogen sequence:
MWRVCARRAQ NVAPWAGLEA RWTALQEVPG TPRVTSRSGP APARRNSVTT GYGGVRALCG WTPSSGATPR NRLLLQLLGS PGRRYYSLPP HQKVPLPSLS PTMQAGTIAR WEKKEGDKIN EGDLIAEVET DKATVGFESL EECYMAKILV AEGTRDVPIG AIICITVGKP EDIEAFKNYT LDSSAAPTPQ AAPAPTPAAT ASPPTPSAQA PGSSYPPHMQ VLLPALSPTM TMGTVQRWEK KVGEKLSEGD LLAEIETDKA TIGFEVQEEG YLAKILVPEG TRDVPLGTPL
Target Information
DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product, dihydrolipoamide acetyltransferase, transfers acetyl groups to coenzyme A. This protein is the antigen for antimitochondrial antibodies, which are present in nearly 95% of patients with primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes destroy epithelial cells in the bile duct where this protein is abnormally expressed, leading to cirrhosis and liver failure.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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