Thermo Fisher Scientific LIG4 Monoclonal Antibody (2D9)
LIG4 단백질을 인식하는 Rabbit monoclonal antibody (Clone 2D9). Western blot 및 IHC(P) 검증 완료. 인간 DNA Ligase IV 타깃, 비결합(Unconjugated) 액상 형태. V(D)J 재조합 및 DNA 복구 연구용. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D9 |
| Immunogen | Recombinant protein within human DNA Ligase IV (aa 500–700) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
Target Information
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). It forms a complex with XRCC4 and interacts with DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene cause LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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