Thermo Fisher Scientific TACC3 Polyclonal Antibody
TACC3 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS와 글리세롤 버퍼에 보관. 인간 시료에 반응하며, 세포 성장 및 분화 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human TACC3. Recombinant protein control fragment (Product #RP-88724). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2648183 |
Product Specific Information
Immunogen sequence:
VEENLSSYSL DRRVTPASET LEDPCRTESQ HKAETPHGAE EECKAETPHG AEEECRHGGV CAPAAVATSP PGAIPKEACG GAPLQGLPGE ALGCPAGVGT PVPADGTQTL
Highest antigen sequence identity to the following orthologs:
- Mouse: 29%
- Rat: 27%
Target Information
TACC1 is located on the 8p11 chromosomal region that is amplified in approximately 15% of all breast tumor samples. The short arm of chromosome 8 also contains FGFR1, whose expression is enhanced in most breast cancer tumors.
TACC family members (TACC1, TACC2, TACC3) map closely to FGFR1, FGFR2, and FGFR3 genes on chromosomes 4, 8, and 10, respectively.
Since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation.
TACC1 contains a conserved C-terminal region similar to the Drosophila homolog D-TACC, which is necessary for normal spindle function. Mammalian TACC proteins appear to interact with centrosomes and microtubules in a similar manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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