Thermo Fisher Scientific Histone H3.3 Recombinant Rabbit Monoclonal Antibody (ST50-08)
Histone H3.3 단백질을 특이적으로 인식하는 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF, ChIP 등 다양한 응용에 적합. HEK293 세포에서 발현된 IgG 항체로, Protein A로 정제됨. 장기 보관 시 -20°C에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ST50-08 |
| Immunogen | Synthetic peptide within human Histone H3.3 (aa 50–100) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Wet ice |
| RRID | AB_2931482 |
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through interaction with linker histone H1, forming higher-order chromatin structures.
The Histone H3.3 gene is intronless and encodes a variant member of the histone H3 family. Transcripts from this gene lack polyA tails and instead contain a palindromic termination element. This gene is located separately from other H3 genes within the histone gene cluster on chromosome 6p22–p21.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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