Thermo Fisher Scientific Phospho-RhoA (Ser188) Polyclonal Antibody
RhoA 단백질의 Ser188 인산화 상태를 검출하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 고순도 Protein A 정제, 안정적인 액상 형태로 -20°C 보관.
- 카탈로그번호
- BS-5330R
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-RhoA (Ser188) Polyclonal Antibody
Thermo Fisher Scientific Phospho-RhoA (Ser188) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human RhoA around the phosphorylation site of Ser188 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal required for myosin contractile ring formation during cytokinesis and plays an essential role in cleavage furrow formation.
RhoA is required for apical junction formation in keratinocyte cell-cell adhesion and acts as a target for Yersinia pestis and Yersinia pseudotuberculosis cysteine peptidase yopT.
It stimulates PKN2 kinase activity and may activate PLCE1. Activated by ARHGEF2, RhoA promotes GDP-GTP exchange and is essential for SPATA13-mediated regulation of cell migration and adhesion assembly/disassembly.
The MEMO1-RHOA-DIAPH1 signaling pathway is crucial for ERBB2-dependent microtubule stabilization at the cell cortex, controlling APC and CLASP2 localization via GSK3B activity regulation. Membrane-bound APC allows MACF1 localization required for microtubule capture and stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 없음)
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.