Thermo Fisher Scientific TOMM22 Monoclonal Antibody (1C9-2)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Immunomicroscopy (IM)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1C9-2

Immunogen

Membrane fraction from Vero (monkey kidney-derived) cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2303718

Product Specific Information

Recommended positive controls: Raji.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Tom22 has a negatively charged N-terminal region exposed to the cytosol, a putative transmembrane region, and a C-terminal intermembrane space region with little negative charge. The following functions can be assigned to the three domains of Tom22: the cytosolic N-terminal domain plays a dual role, specifically important for presequence binding; the intermembrane space domain provides a trans binding site for presequences, and the single membrane anchor of Tom22 is crucial for the integrity of the GIP (general import gene) complex. The TOM complex of mammalian mitochondria resembles the fungal Tom complex, but is distinct from the plant TOM system. Thus, while unique components of the mammalian mitochondrial import system have been identified (e. g. TOM34 and metaxin), Tom22, and Tom37 have not been identified in plant mitochondria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.