Thermo Fisher Scientific TOMM22 Monoclonal Antibody (1C9-2)
TOMM22 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot 및 면역세포화학 실험에 적합합니다. 인간 및 비인간 영장류 시료에 반응하며, PBS/BSA 완충액에 보관됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific TOMM22 Monoclonal Antibody (1C9-2)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 1 publication |
| Immunomicroscopy (IM) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1C9-2 |
| Immunogen | Membrane fraction from Vero (monkey kidney-derived) cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2303718 |
Product Specific Information
- Recommended positive control: Raji cells
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Tom22 has a negatively charged N-terminal region exposed to the cytosol, a putative transmembrane region, and a C-terminal intermembrane space region with little negative charge.
The following functions are assigned to the three domains of Tom22:
- The cytosolic N-terminal domain plays a dual role, specifically important for presequence binding.
- The intermembrane space domain provides a trans binding site for presequences.
- The single membrane anchor of Tom22 is crucial for the integrity of the GIP (general import gene) complex.
The TOM complex of mammalian mitochondria resembles the fungal Tom complex but is distinct from the plant TOM system. Unique components of the mammalian mitochondrial import system (e.g., TOM34 and metaxin) have been identified, while Tom22 and Tom37 have not been identified in plant mitochondria.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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