Thermo Fisher Scientific Transferrin Polyclonal Antibody
Rabbit polyclonal antibody against human and mouse Transferrin. Validated for WB and IHC-P. High specificity and sensitivity with antigen affinity purification. Suitable for detecting iron transport protein Transferrin in various cell lines and plasma ...
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | — |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 339–638 of Human Transferrin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.12 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544782 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, human plasma, mouse plasma
- Predicted reactivity: Chimpanzee (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Transferrins (Tf) are approximately 80 kDa blood plasma glycoproteins synthesized by the liver. They are iron-binding transport proteins capable of binding two Fe(3+) ions with an accompanying anion, typically bicarbonate. Transferrin is responsible for transporting iron from absorption and heme degradation sites to storage and utilization sites. Approximately one-third of total blood transferrin contains bound iron under normal conditions. Measurement of transferrin levels can indicate blood iron-carrying capacity and abnormalities such as anemia, iron overload, and hemochromatosis. Serum transferrin may also promote cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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