Thermo Fisher Scientific Zap-70 Monoclonal Antibody (1E7.2)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 30 publications 30 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1E7.2

Immunogen

GST-fusion protein of Human ZAP70 of amino acids 282-307 [RRIDTLNSDGYTPEPARITSPDKPRP] if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Zap-70,uniProtId:P43403-1,ncbiNodeId:9606,antigenRange:282-307,antigenLength:619,antigenImageFileName:MA1-21350_Zap-70_P43403-1_House_mouse.svg,antigenImageFileNamePDP:MA1-21350_Zap-70_P43403-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.7 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_562001

Product Specific Information

Positive control of Jurkat cell lysate suggested.

Target Information

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.