Thermo Fisher Scientific Zap-70 Monoclonal Antibody (1E7.2)
ZAP-70 단백질을 인식하는 Mouse monoclonal antibody (clone 1E7.2). Western blot, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. Affinity chromatography로 정제된 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | Assay-dependent | View 30 publications |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E7.2 |
| Immunogen | GST-fusion protein of Human ZAP70 (amino acids 282–307: RRIDTLNSDGYTPEPARITSPDKPRP) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.7 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_562001 |
Product Specific Information
Positive control: Jurkat cell lysate suggested.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated at tyrosines 292, 315, and 319 within the interdomain B region between the SH2 and kinase domains. Phosphorylation at tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances signaling, while phosphorylation at tyrosine 292 attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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