Thermo Fisher Scientific ARFGAP1 Monoclonal Antibody (OTI2D5), TrueMAB
ARFGAP1 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody. Western blot, ICC/IF, Flow Cytometry에 적합. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. 막 수송 및 소포 융합 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D5 |
| Immunogen | Full length human recombinant protein of human ARFGAP1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional round of desalting (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ArfGAP1 is a 406-amino-acid regulatory protein containing an Arf-GAP domain that activates the GTPase activity of ADP ribosylation factor (ARF).
It promotes hydrolysis of ARF1-bound GTP and is essential for dissociation of coat proteins from Golgi-derived membranes and vesicles, playing a key role in membrane trafficking and vesicle transport.
The activity of ArfGAP1 is stimulated by phosphoinositides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been reported, and it is ubiquitously expressed in most tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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