Thermo Fisher Scientific Claudin 2 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Immunogen sequence:
WKTSSYVGAS IVTAVGFSKG LWMECATHST GITQCDIYST LLGLPADIQA AQAMMVTSSA ISSLACIISV VGMRCTVFCQ ESRAKDRVAV AGGVFFILGG LLGFIPVAWN LHGILRDFYS PLVPDSMKFE IGEALYLGII SSLFSLIAGI ILCFSCSSQR NRSNYYDAYQ AQPLATRSSP RPGQPPKVKS EFNSYSLTGY V

Target Information

Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell. TJ strands on adjacent cells are presumed to interact with each other to form a sort of "molecular gasket" that prevents ions, water, and other molecules from leaking between cells.

In addition to this “barrier” function, the “fence” function of tight junctions maintains epithelial cell polarity by blocking diffusion of membrane proteins between apical and basolateral surfaces. Several peripheral membrane proteins, such as ZO-1, ZO-2, ZO-3, cingulin, Rab-3b, and symplekin, are associated with tight junctions and are involved in assembly, maintenance, and permeability regulation.

Claudin-1 and claudin-2 are major structural components of tight junction strands, forming networks of strands characteristic of true tight junctions. Claudin-2 functions as a paracellular channel with cation (Na⁺) selectivity and is mainly expressed in liver and kidney, with smaller amounts in brain tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.