Thermo Fisher Scientific Phospho-S6 (Ser244) Polyclonal Antibody
RPS6 단백질의 Ser244 인산화 상태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 고순도 정제된 액상 형태로 안정적인 신호 검출 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human RPS6 (Accession P62753), corresponding to amino acid residues around phosphorylated Ser244 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817433 |
Product Specific Information
Antibody detects endogenous levels of RPS6 only when phosphorylated at Ser244.
Target Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit composed of 4 RNA species and approximately 80 distinct proteins.
This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit and belongs to the S6E family.
RPS6 is a major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different kinases.
Phosphorylation is induced by growth factors, tumor-promoting agents, and mitogens, while dephosphorylation occurs at growth arrest.
The protein may contribute to the control of cell growth and proliferation through selective translation of specific mRNA classes.
As typical for ribosomal protein genes, multiple processed pseudogenes of this gene are dispersed throughout the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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