Applied Biosystems™

AgPath-ID™ One-Step RT-PCR Reagents with manual

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse자세히 알아보기

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
• Recommended for RNA pathogen amplification
• Consistent amplification of RNA targets with high specificity and sensitivity
• Optimized to work with your target-specific primers and probes
• Contains ROX for quantitative fluorescent signal normalization

AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.

사양

설명Works on all AB platforms, but is optimized for the 7500.

검출 방법Primer-probe

형식Tube

GC-Rich PCR PerformanceHigh

PCR 방법1-step RT-qPCR

중합효소AmpliTaq Gold DNA Polymerase

반응 속도Standard

용도(장비)7500 System

패시브 참조 염료ROX (Pre-mixed)

제품라인AgPath-ID™, Ambion™

제품 유형One-Step RT-PCR Reagent

수량1000 Reactions

역전사 효소ArrayScript™

충분1000 Reactions

농도25X

Fidelity (Taq 대비)25 X

반응 수1000 Reactions

부피14 mL

Unit SizeEach