Thermo Fisher Scientific IL-12 p35 Monoclonal Antibody (27537), PerCP

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

10 µL per million cells

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

27537

Immunogen

Sf 21-derived recombinant human IL-12/IL-35 p35 Arg23-Ser219 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-12 p35,uniProtId:P29459-1,ncbiNodeId:9606,antigenRange:23-219,antigenLength:219,antigenImageFileName:MA5-23622_IL-12_p35_P29459-1_House_mouse.svg,antigenImageFileNamePDP:MA5-23622_IL-12_p35_P29459-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PerCP PerCP PerCP

View additional formats

• FITC
• PE
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Purification

Protein A/G

Storage buffer

Buffered saline solution with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609032

Product Specific Information

This antibody detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.

Target Information

Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells. IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.