Thermo Fisher Scientific AKT Pan Recombinant Rabbit Monoclonal Antibody (16E10)
AKT1/2/3 단백질을 인식하는 토끼 유래 리컴비넌트 단클론 항체로, Western blot, IHC, Flow cytometry, ELISA 등 다양한 응용에 적합. 세포 생존 및 신호전달 연구에 활용 가능. 고순도 Affinity chromatography 정제, 안정적 보관 가능.
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- pk
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Applications and Tested Dilutions
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:50–1:200 | - |
| ELISA | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 16E10 |
| Immunogen | A synthesized peptide derived from Human AKT1/2/3 Antibody |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3094351 |
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is a ubiquitous serine/threonine kinase involved in diverse biological responses such as metabolism regulation, cell survival, and growth. It is activated by insulin, PI3K, IGF1, and other growth factors. AKT promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of targets including forkhead transcription factors and caspase-9. The AKT pathway is a major focus in cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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