Thermo Fisher Scientific Vinculin Polyclonal Antibody
Western blot 및 IHC(파라핀)에서 사용 가능한 비결합형 폴리클로날 항체. 사람, 마우스, 랫에 반응하며, Vinculin 단백질의 N-말단 영역을 인식. 세포 부착 및 세포골격 연구용으로 적합. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 12–39 amino acids from the N-terminal region of human Vinculin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Size-exclusion, Dialysis, Ammonium sulfate precipitation |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2214492 |
Product Specific Information
This antibody is predicted to react with chicken, porcine, and rat based on sequence homology.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. Vinculin interacts with talin and alpha-actinin, playing a key role in linking the extracellular matrix to the cytoplasmic microfilament system. Mutations in the VCL gene can result in cardiomyopathy, including dilated type 1W and familial hypertrophic type 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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