Thermo Fisher Scientific Nitrotyrosine Monoclonal Antibody (1F1)

Thermo Fisher Scientific Nitrotyrosine Monoclonal Antibody (1F1)

Applications and Tested Dilution

Product Specifications

Product Specific Information

A suggested positive control for this product is A431 cell lysate treated with 10 mM peroxynitrite.

Target Information

Nitrotyrosine is a relatively stable product formed from various reaction pathways. One notable pathway involves the reaction of peroxynitrite (formed from superoxide and nitric oxide radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins, resulting in the inactivation of certain housekeeping enzymes (e.g., alpha1-antiproteinase) and endogenous antioxidant enzymes such as catalase and superoxide dismutase (SOD).

Nitrotyrosine serves as an indicator of cell damage and inflammation, as well as nitric oxide (NO) production. Measuring nitrotyrosine concentration can be used as a marker for NO-induced cellular damage. It has been implicated in the pathogenesis of several inflammatory, infectious, and degenerative diseases, including Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis, and conditions associated with endothelial injury. At the protein level, tyrosine nitration can lead to loss or alteration of protein function, as demonstrated for p53 and manganese-dependent superoxide dismutase (MnSOD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.