Thermo Fisher Scientific Nitrotyrosine Monoclonal Antibody (1F1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

3 µL

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1F1

Immunogen

Peroxynitrate treated BSA

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_1958973

Product Specific Information

A suggested positive control for this product is A431 cell lysate treated with 10mM peroxynitrite.

Target Information

Nitrotyrosine is a relatively stable product formed from various reaction pathways. Perhaps most notable is the reaction of peroxynitrite (formed from superoxide and nitric oxide (NO) radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins resulting in inactivation of certain housekeeping enzymes (e. g., alpha1-antiproteinase) as well as endogenous antioxidant enzymes such as catalase and superoxide dismutase (SOD). Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Nitrotyrosine has been implicated in the pathogenesis of several inflammatory, infectious, and degenerative human diseases, such as Alzheimers and Parkinsons disease, multiple sclerosis, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis, and a variety of conditions precipitated by endothelial injury. At the protein level, tyrosine nitration can lead to loss or alteration of protein function, as demonstrated for the oncogenic protein p53 and the mitochondrial protein, manganese-dependent superoxide dismutase (MnSOD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.