Thermo Fisher Scientific ERK2 Polyclonal Antibody
ERK2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, Flow Cytometry에 사용 가능. 인간에 반응하며, 액상 형태로 2 mg/mL 농도 유지. MAPK 신호전달 연구 및 세포 내 인산화 경로 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 154–183 amino acids from the central region of human MAPK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Size-exclusion, Dialysis, Ammonium sulfate precipitation |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10982617 |
Product Specific Information
This antibody is predicted to react with bovine, mouse, rat, and Xenopus based on sequence homology.
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as integration points for multiple biochemical signals and participate in various cellular processes such as proliferation, differentiation, transcription regulation, and development. Activation of this kinase requires phosphorylation by upstream kinases. Upon activation, ERK2 translocates to the nucleus of stimulated cells, where it phosphorylates nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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