Thermo Fisher Scientific Dynactin 1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.3-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

6-7 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (QEQLHQLHSRLIS) corresponding to the C-terminus amino acids of DCTN1 (aa 1266-1278). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Dynactin 1,uniProtId:Q14203-1,ncbiNodeId:9606,antigenRange:1266-1278,antigenLength:1278,antigenImageFileName:PA5-18095_Dynactin_1_Q14203-1_Goat.svg,antigenImageFileNamePDP:PA5-18095_Dynactin_1_Q14203-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10982401

Product Specific Information

This antibody is predicted to react with rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

Target Information

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.