Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Alexa Fluor 561, eBioscience

Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Alexa Fluor 561, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 647
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 563 / 615
• Brilliant Violet 421 / 480 / 605 / 711
• eFluor 450 / 506
• FITC
• Functional Grade
• Pacific Orange
• PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The 2F1 monoclonal antibody reacts with mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA).
KLRG1 is a homodimer of glycosylated 30–38 kDa subunits and contains a cytoplasmic motif similar to ITIM. It is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with Ly49 molecules and SHP-1 signaling.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry of mouse splenocytes; ≤0.25 µg/test recommended.
A test is defined as the amount of antibody staining a 100 µL cell sample (10⁵–10⁸ cells/test). Titrate for optimal performance.

Note: Alexa Fluor 561 may non-specifically stain B cells in Swiss Webster and SJL mice; not observed in BALB/c or C57BL/6 strains.

Spectral Information:

• Excitation: 558 nm
• Emission: 575 nm
• Laser: Yellow-Green Laser
  Alexa Fluor 561 emits at 575 nm and is suitable for spectral cytometers; multiplexing possible with PE and PE-eFluor 610.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a lectin-like type 2 transmembrane receptor containing ITIMs that interact with SH2 domains of phosphatases such as SHP-1.
It acts as an inhibitory receptor expressed on NK cells and certain T cells, involved in tumor and virus-infected cell lysis and immune regulation.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.

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