Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Alexa Fluor 561, eBioscience

Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Alexa Fluor 561, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test

Product Specifications

Available Formats

• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 647
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 563 / 615
• Brilliant Violet 421 / 480 / 605 / 711
• eFluor 450 / 506
• FITC
• Functional Grade
• Pacific Orange
• PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The 2F1 monoclonal antibody reacts with mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA).
KLRG1 is a homodimer of glycosylated 30–38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM).
This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test.
A test is defined as the amount of antibody staining a cell sample in 100 µL volume. Cell number may range from 10⁵–10⁸ cells/test.
Careful titration is recommended for optimal performance.

Fluorophore Information:

• Alexa Fluor 561 emits at 575 nm
• Excitation: 558 nm
• Laser: Yellow-Green
• Suitable for multiplexing with PE and PE-eFluor 610 on spectral cytometers

Internal testing indicates strain-dependent non-specific staining of B cells in Swiss Webster and SJL mice, not observed in BALB/c or C57BL/6 strains.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family.
It contains ITIM motifs that interact with SH2 domains of phosphatases such as SHP-1.
KLRG1 is expressed on NK cells and certain T cells, functioning as an inhibitory receptor involved in the regulation of immune responses against tumor and virus-infected cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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