Thermo Fisher Scientific Ku80 Monoclonal Antibody (S.669.4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:400-1:1,600

-

Immunocytochemistry (ICC/IF)

1:200-1:800

-

Immunoprecipitation (IP)

1:50

-

ChIP assay (ChIP)

2.5 µg/10^6 cells

-

in situ PLA (PLA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

S.669.4

Immunogen

Synthetic peptide corresponding to the carboxy terminus of human Ku80 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ku80,uniProtId:P13010-1,ncbiNodeId:9606,antigenRange:732,antigenLength:732,antigenImageFileName:MA5-14953_Ku80_P13010-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14953_Ku80_P13010-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

7 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10979323

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.