Thermo Fisher Scientific HCN1 Polyclonal Antibody
HCN1 단백질을 인식하는 Rabbit Polyclonal 항체로, 인간, 마우스, 랫트에 반응합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS와 20% 글리세롤 완충액에 보관됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View publication |
| Immunohistochemistry (IHC) | - | View publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human HCN1. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.45 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735891 |
Product Specific Information
- Positive Control: SK-N-AS, mouse brain, rat brain
- Predicted Reactivity: Rabbit (90%), Bovine (89%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HCN1 (Hyperpolarization-activated cyclic nucleotide-gated channel 1) belongs to the HCN gene family, which contributes to spontaneous rhythmic activity in both heart and brain. HCN1 channels are activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play key roles in motor learning and neuronal integration by cerebellar Purkinje cells, influencing the autonomous activity of single neurons and the rhythmicity of network oscillations.
HCN1 expression is enriched in the cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is abundant in mammillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. Variations in regional HCN channel expression contribute to differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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