Thermo Fisher Scientific CD11b Monoclonal Antibody (VIM12), TRI-COLOR

Applications

• Flow Cytometry (Flow)

Tested Dilution: Assay-Dependent
Publications: - References

---

Product Specifications

---

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• FITC
• PE
• Request custom conjugation

---

Product Specific Information

The TRI-COLOR® conjugate is a tandem R-phycoerythrin (PE)-Cy5 conjugate that allows simultaneous multicolor labeling and detection of multiple targets using a single excitation source—the 488 nm line of the argon-ion laser.

---

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3).

CD11b is expressed on:

• Monocytes/macrophages
• Granulocytes
• Activated lymphocytes
• Subsets of NK cells and dendritic cells
• Microglia in the brain

Functions as receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. These adhesions are critical for cell-cell and cell-matrix interactions.

Expression levels:

• 8% of spleen cells
• 44% of bone marrow cells
• <1% of thymocytes

Commonly used as a microglial marker in nervous tissue. Expression increases during monocyte maturation and varies among tissue macrophages. Peritoneal macrophages express higher levels of CD11b than splenic macrophages.

Diseases associated with CD11b dysfunction include systemic lupus erythematosus and ITGAM-related susceptibility to systemic lupus erythematosus.

---

Analyte Specific Reagent

---

제품 이미지