Thermo Fisher Scientific Glu-Glu Tag Polyclonal Antibody
Thermo Fisher Scientific의 Glu-Glu Tag Polyclonal Antibody는 Glu-Glu(EYMPME) 태그 단백질 검출용 항체입니다. Western blot 및 ICC/IF에 적합하며, 항원 친화 크로마토그래피로 정제된 Rabbit IgG 폴리클로날 항체입니다. 액상 형태로 제공되며, -20°C에서 보관합니다.
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Thermo Fisher Scientific Glu-Glu Tag Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:400 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 129 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10982314 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The Glu-Glu tag, also known as the EYMPME sequence, is a short peptide epitope tag consisting of two glutamic acid (Glu) residues interspersed with various amino acids. It is typically incorporated into the N-terminus of proteins through subcloning into vectors such as the pFASTBAC vector. This tag is useful in recombinant protein expression and purification processes, facilitating detection and isolation of tagged proteins via specific antibodies recognizing the Glu-Glu sequence. The Glu-Glu tag can be used in systems including baculovirus expression in insect cells, providing efficient means for studying protein-protein interactions, localization, and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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