Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Blue 690, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.4 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet/Yellow series
• eFluor 450, 506, 660
• FITC
• PE, PE-Cyanine series
• PerCP-Cyanine5.5
• Super Bright series
• NovaFluor Blue, Red, Violet, Yellow series
• Custom conjugation available

Product Specific Information

Description:
The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except plasma cells. Follicular dendritic cells also express CD19. CD19 forms a complex with CD21, CD81, and MHC class II, associating with the BCR to mediate signaling that induces tyrosine phosphorylation, calcium flux, and proliferation.

Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls, add 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Technology:
Based on Phiton™ technology utilizing nucleic acid dye structures, engineered for precise fluorescent signatures with minimal spillover and spread.
Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and some myeloid leukemia cells. CD19 serves as a key regulator of B cell activation, proliferation, and differentiation. It lowers the activation threshold for antigen receptor signaling and acts as a signal-amplifying coreceptor for the BCR. Mutations in CD19 can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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